关键词: 酒精; 缺血-再灌注; 梗死面积; 凋亡诱导因子; 缺氧诱导因子-1&alpha

Abstract:

【Abstract】 Objective To investigate the influence of alcohol on the infarction area and expression of apoptosis induced factor(AIF), hypoxia induced factor-1α(HIF-1α) in the brain tissue of rats with cerebralreperfusion. Methods Fifty-four healthy male Wistar rats were randomly divided into 3 groups, sham operation group, ischemia-reperfusion group(control group), and ischemia-reperfusion with alcohol group(therapy group). The infarction models were established by blocking the middle cerebral artery, after 2 hours the line was pulled out, forming ischemia-reperfusion in the brain tissue of the rats. Alcohol(absolute alcohol was diluted to 50% alcohol) 1.5 g/kg was intraperitoneally injected to the therapy group when reperfusion, and the control group and sham operation group with the same amount of saline. The infarct size was stained by 2, 3, 5-triphenyltetrazolium chlorice and the expression of AIF and HIF-1α in the brain tissue of the rats was tested by the immunohistochemical method. Results The infarction area in the therapy group was significantly smaller than that in the control group([35.33 6.06]mm2 vs [55.50 3.62]mm2, P <0.001). The expression of AIF and HIF-1α in the brain cortex of rats was (36.75 8.99)/HP and (49.25 12.04)/HP in the control group and (20.75 7.46)/HP and (70.25 11.12)/HP in the therapy group, both of them were higher than the sham operation group(both P <0.001), but the expression of AIF in the brain cortex of rats in the therapy group was significantly lower(P <0.001) and the expression of HIF-1α was significantly higher than the control group(P <0.001).

Conclusion Alcohol(1.5 g/kg) has neuroprotective effects on brain tissue of rats with ischemiareperfusion. In brain ischemia -reperfusion injury, alcohol may reduce the brain damage by the enhancement of nerve cells to adapt the anaerobic environment and reducing apoptosis of the nerve cells.

Key words: Alcohol; Cerebral-reperfusion; Infarct area; Apoptosis induced factor; Hypoxia induced factor-1α