关键词: 大鼠; 海马; 齿状回; 溴脱氧尿嘧啶核苷; 巢蛋白

Abstract: Objective To explore the effect of behavior training on the proliferation of neural stem cells in the dental gyrus (DG) of hippocampus injury-infarcted rats.Methods One hundred and eight Sprague-Dawley rats were randomized into behavior training group (1, 7, 14, 21, 28 and 35 days), no-training group (1, 7, 14, 21, 28 and 35 days) and control group (1, 7, 14, 21, 28 and 35 days). Photochemical initiation was used to induce hippocampal injuryinfarctionin training and no-training group. Morris water maze training or no-training was performed 1 day after surgery. Double staining immunofluorescence was used to detect the expression of BrdU/Nestin in the dental gyrus (DG) at different time points.Results Few BrdU/Nestin double staining cells were observed in the DG of control rats. In the behavior training and no-training groups for 7, 14, 21 and 28 days, the number of BrdU/Nestin double-stained cells increased in the DG on the opposite side compared with the control group (P <0.01). BrdU/Nestin double-stained cells increased obviously after 7, 14, 21 and 28 days in thebehavior training group compared with the no-training group(P <0.01). In the behavior training and no-training groups for 35 days, no significant difference of the number of BrdU/Nestin double-stained cells in the DG on the opposite side was found between them and the control group (P >0.05).Conclusion Behavior training can accelerate the proliferation of neural stem cells and then encourage the recovery of neural function.

Key words: Rats; Hippocampus; Dentate gyrus; BrdU; Nestin