关键词: 脑脊液净化仪; 净化; 红细胞; 临床实验室技术

Abstract: Objective To evaluate the stability, efficiency and load capacity of cerebrospinal fluid (CSF)purification system and to provide experimental data for further clinical trial through the purificationexperiment for simulative blooded cerebrospinal fluid in vitro.Methods 2.5%, 5% and 7.5% simulative blooded CSF were prepared using physiological salinewith preserved red blood cells from healthy volunteers. CSF purification system was developedwith national patent. According to the purification parameters of normal CSF circulation in vivo,those simulative blooded CSF were purified in vitro using CSF purification system. The number ofred blood cells was analyzed before and after purification.Results In the experiment to determine the maximum load capacity of filter membrane, 330ml offluid circulation were needed in order to achieve more than 90% red blood cell (RBC) removingrate for 2.5% simulative blooded CSF, and 390ml, 420ml were needed separately, for 5% and 7.5%.For more than 95% RBC removing rate, 390ml, 450ml and 480ml of fluid circulation were requiredfor 2.5%, 5% and 7.5%, separately. By six-round purification circulation using a single set of filtermembrane, RBC counts in 2.5% simulative blooded CSF decreased to 63 900/ mm3 from 153 000/mm3 and the decline rate exceeded 55%.Conclusion CSF purification system is able to remove RBC effectively from simulative bloodedCSF with high stability.

Key words: Cerebrospinal fluid purification system; Decontamination; Erythrocytes; Clinicallaboratory techniques