Potassium Aspartate Alleviates Brain Injury Induced by Controlled Cortical Impact in Rats

doi:10.3969/j.issn.1673-5765.2017.06.005

Abstract

Abstract:

Objective Potassium aspartate (PA), as an electrolyte supplement, is widely used in clinical practice. Previous study found PA had neuroprotective effects against apoptosis after cerebral ischemia/reperfusion in rats. This study is to investigate whether PA has protective effects on traumatic brain injury. Methods Traumatic brain injury (TBI) was induced by controlled cortical impact (CCI) in rats. Vehicle treatment group or potassium aspartate treatment group were administered saline via intraperitoneal injection or PA at the dose of 62.5 mg/kg at 30 minutes after onset of CCI. The brain blood perfusion, modified Neurological Severity Score (mNSS), and cortical lesion volume were observed. Brain edema brain ATP contents, lactic acid concentration, and Na+/K+-ATPase activities were measured. Results In controlled cortical impact induced cortical injury in rats, compared with vehicle-only treatment, PA treatment during the acute phase of MCAO at the dose of 62.5 mg/kg significantly improved neurological deficits (P <0.05), decreased the cortical lesion volume (P <0.01), and alleviated brain edema (P <0.05). In addition, PA treatment significantly reduced the loss of ATP (P <0.01), reduced lactic acid levels (P <0.05), and increased the activity of Na+/K+-ATPase (P <0.05). Conclusion PA had neuroprotective effects on TBI through increasing ATP, Na+/K+-ATPase activity, and reducing brain edema. It provides experimental evidence for the clinical application of PA in clinical treatment of brain injury.

Key words: Aspartic acid; Brain injury; Controlled cortical impact; Ischemia; Na+/K+-ATPase

摘要：

目的门冬氨酸钾（potassium aspartate，PA）作为一种电解质补充剂，在临床上广泛使用。以往的研究发现门冬氨酸钾在脑缺血/再灌注大鼠中对细胞凋亡有神经保护的作用。本研究将探讨门冬氨酸钾对创伤性脑损伤（traumatic brain injury，TBI）是否有保护作用。方法 T BI通过大鼠可控性皮质打击伤（controlled c ortical i mpact，CCI）产生。门冬氨酸钾组或溶剂对照组在CCI发生后30 min以腹腔注射给予生理盐水或62.5 mg/kg剂量的PA，观察脑血流灌注量，改良神经功能缺损评分（modified Neurological Severity Score，mNSS）和皮质损伤体积，并检测脑水肿以及脑组织三磷腺苷（adenosine triphosphate，ATP）、乳酸含量和钠钾ATP酶活性。结果在CCI引起的大鼠皮质损伤中，与溶剂对照组相比，急性给予62.5 mg/kg剂量的PA治疗可以明显改善神经功能缺损（P＜0.05)，降低皮质损伤体积（P＜0.01），减轻脑水肿（P＜0.05）。此外，与溶剂对照组相比，门冬氨酸钾治疗组显著减少ATP缺失（P＜0.01），降低乳酸含量（P＜0.05），并增加钠钾ATP酶的活性（P＜0.05）。结论 PA能通过增加ATP含量和钠钾ATP酶的活性并降低脑水肿，对TBI具有神经保护作用。这为PA 在临床脑损伤时的应用提供了实验证据。

关键词: 门冬氨酸钾; 脑损伤; 可控性皮质打击伤; 钠钾ATP酶

GU Yi,ZHAO Yu-Mei, SU Yu-Jin. Potassium Aspartate Alleviates Brain Injury Induced by Controlled Cortical Impact in Rats[J]. Chinese Journal of Stroke, 2017, 12(06): 489-495.

顾漪，赵育梅，苏玉金. 门冬氨酸钾减轻大鼠可控性皮质打击伤引起的脑损伤[J]. 中国卒中杂志, 2017, 12(06): 489-495.

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