Effect of Ginkgo Biloba Extract-EGb 761 on Beta Sceretase of Rat Hippocampal
LIU Wei;ZHANG Xiang-Jian;HU Ming;et al.
2008, 3(08):
0-586.
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Objective To observe the expression of insulin-like growth factor-1(IGF-1) and the change ofhistopathology in the ischemic region of rats with focal cerebral ischemia-reperfusion, so as to explorethe neuron protection of IGF-1.Methods All the rats were divided into sham group and cerebral ischemia-reperfusion(CIR) grouprandomly, 36 rats for each group. After ischemia for 3h, according to the time of reperfusion(0h,6h,24h,48h,72h and 7d), the CIR group and sham group were both divided into 6 subgroups and everysubgroup was 6 rats. The neurological deficit score(NDS) was assessed with 3 different methods, andhistopathology changes were observed with hematoxylin and eosin(HE) staining. We observed theexpression of IGF-1 by immunohistochemistry.Results ①The ethology score increased after the operation and at 24 to 72h after reperfusion thescores of CIR group were more than those of sham group. At 7d after reperfusion the ethology scorewas almost normal in CIR group. ②In the CIR group, we found the inflammatory cell infiltration inthe infarction regions with the peak at 48h，and continued to 7d. ③The expression of IGF-1 was verylow in normal tissues, while increased mainly in infarction regions after cerebral ischemia. In CIRgroup the expressions of IGF-1 protein were 147±5, 153±6, 170±7, 169±5, 150±7, 147±5 at the timeof reperfusion(0h, 6h, 24h, 48h, 72h and 7d) respectively. At 0h and 6h after reperfusion the positivecells expressed IGF-1 in central and penumbra region increased and with the time prolonged，theexpressions were normal in the central region but still increased in penumbra region, and reachedthe peak at 24 h and 48h after reperfusion. At 72h after reperfusion the expression of IGF-1 proteindecreased but was also higher than that of sham group.Conclusion Enhanced expression of endogenous IGF-1 has protective effects on focal cerebralischemia reperfusion injury.
