【Abstract】
Objective To investigate the influence of alcohol on the infarction area and expression of apoptosis
induced factor(AIF), hypoxia induced factor-1α(HIF-1α) in the brain tissue of rats with cerebralreperfusion.
Methods Fifty-four healthy male Wistar rats were randomly divided into 3 groups, sham operation
group, ischemia-reperfusion group(control group), and ischemia-reperfusion with alcohol
group(therapy group). The infarction models were established by blocking the middle cerebral
artery, after 2 hours the line was pulled out, forming ischemia-reperfusion in the brain tissue of the
rats. Alcohol(absolute alcohol was diluted to 50% alcohol) 1.5 g/kg was intraperitoneally injected
to the therapy group when reperfusion, and the control group and sham operation group with the
same amount of saline. The infarct size was stained by 2, 3, 5-triphenyltetrazolium chlorice and the
expression of AIF and HIF-1α in the brain tissue of the rats was tested by the immunohistochemical
method.
Results The infarction area in the therapy group was significantly smaller than that in the control
group([35.33 6.06]mm2 vs [55.50 3.62]mm2, P <0.001). The expression of AIF and HIF-1α in
the brain cortex of rats was (36.75 8.99)/HP and (49.25 12.04)/HP in the control group and
(20.75 7.46)/HP and (70.25 11.12)/HP in the therapy group, both of them were higher than the
sham operation group(both P <0.001), but the expression of AIF in the brain cortex of rats in the
therapy group was significantly lower(P <0.001) and the expression of HIF-1α was significantly
higher than the control group(P <0.001).
Conclusion Alcohol(1.5 g/kg) has neuroprotective effects on brain tissue of rats with ischemiareperfusion.
In brain ischemia -reperfusion injury, alcohol may reduce the brain damage by the
enhancement of nerve cells to adapt the anaerobic environment and reducing apoptosis of the nerve
cells.
