Abstract: Objective To investigate the effects of lipoic acid on triglyeride(TG), total cholesterol(TC), and the activities of superoxide dismutase(SOD) and glutathione peroxidase (GSH-PX) in cultured vascular smooth muscle cells(VSMC) induced by ox-LDL.Methods Twenty special pathgon free and male SD rats were sacrificed and isolated their thoracic aorta VSMC. VSMC was divided into five groups: control, ox-LDL, ox-LDL with different lipoic acid (20,80,100&#8197;mg/L) and the control group donot were gave any drugs, and four experiment groups were exposed to ox-LDL and then incubated with different concentrations of lipoic acid respectively. The levels of TG and TC were measured and the activities of SOD and GSH-PX were detected in VSMC.Results It were found that the levels of TG, TC in ox-LDL group was significantly higher than those in the control group(P<0.05), the activities of SOD and GSH-PX were much lower than those in the control group(P<0.05). Lipoic acid could significantly lower the levels of TG, TC(P<0.05) and enhance the levels of SOD and GSH-PX(P<0.05) in VSMC induced by ox-LDL.Conclusion Lipoic acid can inhibit lipid accumulation in VSMC induced by ox-LDL. The mechanisms may be associated with the increase of self-anti-oxidation ability of lipoic acid.

Key words: Lipoic acid(LA); ox-LDL; Foam cell; Myocytes; smooth cells

摘要： 目的 观察硫辛酸（LA）对氧化低密度脂蛋白（ox-LDL）诱导的大鼠胸主动脉平滑肌细胞（VSMC）内甘油三脂(TG)、总胆固醇(TC)、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）活性的影响。方法 选取SPF级、雄性SD大鼠20只，安乐死后分离胸主动脉血管中膜，将获得的VSMC分为5组:空白对照组、ox-LDL组、ox-LDL＋LA（20、80、100&#8197;mg/L）组。空白对照组不加任何药物，各实验组以ox-LDL为泡沫细胞诱导剂，加入不同浓度的LA，分别测定VSMC内TG、TC、SOD和GSH-PX的活性。结果 ox-LDL组TG、TC、SOD和GSH-PX值与空白对照组比较差异有统计学意义（P<0.05）；LA各浓度干预组（20、80和100&#8197;mg/L）TG、TC、SOD和GSH-PX值与ox-LDL组比较差异有统计学意义（P<0.05）,各LA剂量组之间比较差异有统计学意义（P<0.05）。结论 LA可抑制ox-LDL诱导VSMC的脂质堆积，抑制泡沫细胞的形成，这可能与其抗氧化活性有关。

关键词: 硫辛酸; 氧化低密度脂蛋白; 泡沫细胞; 肌细胞; 平滑肌