Abstract:

Objective To observe the effect of panax notoginseng saponins (PNS) on expression of apoptosis inducing factor (AIF) in hippocampus of rats with cerebral ischemia-reperfusion, and to investigate the mechanism of neuroprotective effect of PNS. Methods A total of 68 male Wista rats were randomly divided into sham operation group, normal saline control group and PNS treatment group, respectively. The saline control group and each PNS group were re-divided into ischemia-reperfusion 0 h, 2 h, 4 h, 6 h subgroups according to the intervention time points. The modified suture method was used to complete the rat middle cerebral artery occlusion (MCAO) model preparation. The suture was pulled out 2 h later, to cause formation of ischemia-reperfusion. After MCAO 2 h reperfusion at 0 h, 2 h, 4 h, 6 h point in time, intraperitoneal injection of low dose PNS (50 mg/kg), moderate dose PNS (100 mg/kg) and high dose of PNS (150 mg/kg) were given to the PNS treatment group; equivalent-volume of normal saline were given to the control groups, respectively. Immunohistochemistry assay was used to detect the expressions of AIF in the hippocampus of ischemic sides in ischemia-reperfusion rat. Results Compared to sham operation group, the number of AIF positive cells in the saline control group and PNS group significantly increased (All P <0.01). Compared to the saline control group, the number of AIF positive cells in PNS group significantly decreased (All P <0.01). Compared to low and moderate dosage group in PNS group, the number of AIF positive cells in high dose group

significantly decreased (All P <0.01). Conclusion The PNS may decrease hippocampus AIF expression in cerebral ischemia–reperfusion rat, which maybe have protective effect through the inhibition of AIF expression.

Key words: Cerebral ischemia-reperfusion; Apoptosis inducing factor; Panax Notoginseng Saponins; Rat

摘要：

目的 观察三七总皂甙（panax notoginseng saponins，PNS）对脑缺血-再灌注大鼠海马区脑组织凋亡诱 导因子（apoptosis inducing factor，AIF）表达的影响，探讨PNS神经保护作用机制。 方法 68只雄性Wistar大鼠分为假手术组、生理盐水对照组（对照组）和PNS干预组（干预组），再按照 干预时间点将对照组与干预组分为缺血-再灌注O h、2 h、4 h、6 h亚组。改良线栓法制备大鼠大脑中动 脉闭塞模型（middle cerebral artery occlusion，MCAO），2 h后拔出栓线，造成缺血-再灌注。依次于再灌注 后0 h、2 h、4 h、6 h分别对干预组大鼠腹腔内注射小剂量PNS（50 mg/kg）、中剂量PNS（100 mg/kg）和 大剂量PNS（150 mg/kg），对照组注射等体积生理盐水。采用免疫组化法检测大鼠脑缺血-再灌注缺 血侧海马区脑组织AIF的表达并对比各组的情况。 结果 假手术组大鼠海马区脑组织AIF阳性细胞表达数显著少于对照组和PNS干预组各亚组（均 P <0.01）；PNS干预组相同干预时间点的不同剂量组脑组织AIF阳性细胞表达数均明显少于生理盐水 对照组（均P <0.01），与PNS小剂量组和中剂量组相比，PNS大剂量组AIF阳性细胞数表达显著减少。 结论 P NS可使缺血-再灌注大鼠缺血侧海马区脑组织AIF表达减少，可能通过抑制AIF表达而起到神 经保护作用。

关键词: 脑缺血-再灌注; 凋亡诱导因子; 三七总皂甙; 大鼠