关键词: 蛛网膜下腔出血; 迟发性脑血管痉挛; 氧合血红蛋白; 动脉血; 静脉血

Abstract:

Objective To observe the effect of different concentrations of oxyhemoglobin on delayed cerebral vasospasm (DCV) after subarachnoid hemorrhage (SAH) in rats. Methods 24 Sprague-Dawley (SD) rats were divided into three groups: control group (n =8), arterial blood SAH group (n =8) and venous blood SAH group (n =8). SAH models were established by the following methods: 0.3 mL normal saline, 0.3 mL autogenous arterial blood and 0.3 mL autogenous venous blood were injected into the cisterna magna with double injection of blood, respectively. After 7 days, the swelling and blood clots around basilar arteries, wall area and wall thickness of basilar artery , and the ultrastructural changes were observed among the three groups. Results Some blood clots around basilar arteries and brain swelling were observed in arterial and venous blood SAH groups. Under the light microscope, compared with the control group, basilar artery wall area was reduced and vessel wall was thickened in arterial and venous blood SAH groups; compared with venous blood SAH group, basilar artery wall area was reduced (40 816.09±10 410.51 nm2 vs 68 480.89±12 687.4 nm2, P <0.001), the vessel wall was thickened in arterial blood SAH group (3.07±0.59 μm vs 2.36±0.25 μm, P =0.007). The disordered smooth muscle cells and endothelial cells vacuolar degeneration were observed under electron microscope in arterial and venous blood SAH groups, both of which were more worse in arterial blood SAH group than in venous blood SAH group. Conclusions The DCV after SAH induced by different concentrations of oxyhemoglobin can be simulated by injecting autogenous arterial and venous blood. Reducing the concentration of oxyhemoglobin can attenuate DCV after SAH.

Key words: Subarachnoid hemorrhage; Delayed cerebral vasospasm; Oxyhemoglobin; Arterial blood; Venous blood