关键词: 基质金属蛋白酶-3; 基因多态性; 颈动脉狭窄

Abstract: Objective To detect a possible association between Matrix metalloproteinase-3 (MMP-3) promoter-1612 5A/6A polymorphisms and increased risk of carotid stenosis. To investigate the mechanisms by which MMP-3 polymorphisms are involved by examining serum concentrations of MMP-3.Methods A total of 421 carotid-artery stenosis patients who underwent carotid B-mode ultrasonography and control subjects without significant sternosis were selected into the study. The MMP-3 genotype was determined by polymerase chain reaction-restriction fragment length Polymorphism (PCR-RFLP) and the serum MMP-3 concentration was quantified with enzyme-linked immunosorbent assay (ELISA).Results The 6A/6A genotype was associated with carotid sternosis (OR=2.55, 95%CI 1.07-6.07, P=0.026), and the frequency of the 6A allele was significantly higher in patients with carotid sternosis (OR=1.58, 95%CI 1.08-2.33, P=0.014). Carotid sternosis patients had higher serum MMP-3 concentration than controls (19±9 vs 16±7μg/L, P<0.01). There was a strong association between MMP-3-1612 5A/6A genotype and serum concentrations of MMP-3.Conclusion MMP-3 promoter -1612 5A/6A polymorphisms are associated with carotid stenosis, and 6A allele predicts an increased risk of carotid stenosis, in which the increased serum concentration of MMP-3 is involved.

Key words: Matrix metalloproteinase 3; Genetic polymorphism; Carotid stenosis