›› 2010, Vol. 5 ›› Issue (10): 833-835.

• 论著 • 上一篇    下一篇

改良Movat技术在动脉粥样硬化研究中的应用

赵莉红,贺志立,滕梁红,汤国才,卢德宏   

  1. 北京市首都医科大学宣武医院病理科
  • 收稿日期:2010-03-02 修回日期:2010-02-02 出版日期:2010-10-20 发布日期:2010-10-20
  • 通讯作者: 卢德宏

The Application of Modified Movat Pentachrome Stain in the Study of Atherosclerosis

ZHAO Li-Hong, HE Zhi-Li, TENG Liang-Hong, et al.   

  • Received:2010-03-02 Revised:2010-02-02 Online:2010-10-20 Published:2010-10-20
  • Contact: LU De-Hong

摘要: 目的 探索一种改良的染色方法,弥补传统Movat五色套染技术中Resorcin-Fuchsin方法显示动脉的弹力内膜不够完整的缺陷。方法 从尸检病例中选取有不同程度粥样硬化的弹性动脉和肌性动脉各6段,常规制作石蜡蜡块,并进行HE染色。更换Movat套染中的Weigenrt弹力纤维染色法,改用Verhoeff方法来显示动脉中的弹力纤维。结果 弹力纤维染成黑色,蛋白聚糖呈蓝色,胶原纤维染呈黄色,平滑肌纤维呈红色,纤维蛋白和红细胞呈鲜红色。结论 改良后的Movat技术能更清楚的显示动脉组织结构和动脉粥样硬化斑块的形态。

关键词: 动脉粥样硬化; Movat技术; 病理学; 临床

Abstract: Objective To explore a modified method of dyeing to improve the defect of Weigert's resorcinfuchsinstaining in incomplete staining of intimal elastic lamina.Methods Six segments of each elastic and muscular arteries were selected from the autopsycases of atherosclerosis in varying degrees. Routin paraffin section techniques and HE stain wereperformed on these tissues. Verhoeff method was applied to show elastic fibers in the arteriesinstead of Weigert's resorcin-fuchsin stain in the Movat pentachrome stain.Results Elastic fibers, proteoglycans, collagen fibers, smooth muscle fibers and, fibrin/red bloodcells were stained black, blue, yellow, red, and bright red respectively.Conclusion Modified Movat pentachrome stain can show both the artery structure andatherosclerotic plaque morphology more clearly.

Key words: Atherosclerosis; Movat pentachrome stain; Pathology; clinical; modifiied