Abstract: Objective To explore the effects of human adipose-derived stem cells(hASCs) on cognitiveimpairment induced by chronic cerebral hypoperfusion in rats.Methods Adipose-derived stem cells(ASCs) were isolated, cultured, and expanded from humanadipose tissue in vitro. Chronic cerebral hypoperfusion was induced using the two-vesselocclusion (2VO) model by permanent bilateral carotid arteries ligation(BCAL) in rats. AfterBCAL, intravenous infusion of 2×106/ml of hASCs was administered at 1 day, 1 week and 3 weeksrespectively. Control groups were injected with 0.9% saline. Water maze test was performed 5weeks after BCAL for oberseving effects of hASCs at different time points on cognitive impairmentinduced by chronic cerebral hypoperfusion in rats.Results Swimming time of 2VO rats were significantly different from sham-operation group(P <0.01). The improvement of cognitive deficits was found in 2VO rats infused with hASCs at 3weeks treatment compared with saline control group. The outcome of 1 day and 1 week groups donot have statistical significance.Conclusion Intravenous infusion of hASCs can improve the cognitive impairment of chroniccerebral hypoperfusion in rats, and the effect of the chronic phase is better than the acute phase.

Key words: Adipose-derived stem cells; Humans; Brain ischemia; Cognition impairment

摘要： 目的 观察人类脂肪干细胞（human adipose-derived stem cells，hASCs）对慢性脑缺血大鼠认知功能障碍的影响。方法 体外分离、培养及扩增hASCs；利用结扎双侧颈总动脉的方法制备慢性前脑缺血大鼠模型（two-vessel occlusion model，2VO模型），分别于造模后1d、1周、3周经尾静脉注射hASCs（2×106/ml）。造模5周后进行行为学实验，观察缺血后不同时间点应用hASCs对大鼠认知功能障碍的影响。结果 模型组的游泳时间明显长于假手术组（P <0.01）；造模后3周给予hASCs对模型组大鼠的认知功能障碍有改善作用（P <0.05），而造模后1d及1周组的结果无统计学意义（P >0.05）。结论 静脉给予hASCs可改善慢性脑缺血大鼠的认知功能，且慢性期治疗效果较急性期好。

关键词: 脂肪干细胞; 人类; 脑缺血; 认知障碍