关键词: 梗死; 大脑中动脉; 高压氧; 血管内皮生长因子

Abstract: Objective To assess the effect of durative-9-hour hyperbaric oxygenation (HBO) on cerebral infarct volume in the treatment of rats with permanent middle cerebral artery occlusion (MCAO) in superearly stage.Methods Using the intraluminal suture method, we subjected male Sprague-Dawley rats topermannent MCAO model, then the rats were randomly divided into control group and HBO group.Three hours after MCAO, the rats in the HBO group stayed in the hyperbaric cabin with a pressure of 0.2Mpa for 9 hours. Thirteen hours and 5d after MCAO we calculated the cerebral infarct volume with triphenyltetrazolium chloride(TTC) staining and measured the expression of vascular endothelial growth factor (VEGF) using immunohistochemical staining.Results (1) On 13h, there was no significant difference in the infarct volume between the two groups(P >0.05), and at 5d the volume of control group was larger than that on 13h(P <0.05), while in HBO group the volume was smaller than that in control group (P <0.05). (2) The expression of VEGF increased in both groups on 13h point, and there was no significant difference between the two groups(P >0.05). At 5d after MCAO, the expression of VEGF in control group decreased significantly compared with that on 13h point(P <0.01), but in HBO group the expression of VEGF increased significantly when compared with that on 13h point and that in control group at 5d point(P <0.01).Conclusion Durative-9h HBO protocol in super-early stage can reduce the volume of cerebral infarction in rats. Stimulating more production of endogenous VEGF may be one of the mechanisms.

Key words: Infarction; Middle cerebral artery; Hyperbaric oxygenation; Vascular endothelialgrowth factor