Abstract: Objective To establish the rat model of post-stroke depression (PSD).Methods The focal cerebral ischemia model was set up by blocking the middle cerebral artery (MCA). Then the model rats were separately raised and put into chronic unpredictable mild stress (CUMS) to induce the PSD model and some of them were intervened by fuoxertine. The rats were divided into control, stroke, depression, post-stroke depression and PSD treated with fuoxertine groups and all of them were examined dynamically by Open-feld test (OFT), sucrose consumption test and forced swimming test.Results The animal models of PSD had significantly less weight gain than control group and stroke group(P<0.01)at day 14 after CUMS. After given fuoxertin the animals' body weight in PSD group increased signifcantly than before. The scores of horizontal and vertical movement activities in OFT of PSD group were signifcantly less than in control and stroke groups(P<0.05 or P<0.01). The fxed time in forced-swimming test was signifcantly longer in PSD group when compared with control and stroke groups(P<0.05). Fluoxertin markedly increased open-feld activities(P<0.05 or P<0.01) and decreased the fxed time in forced-swimming test(P<0.01).Conclusion Anhedonia and underactivity, the core symptoms in the PSD patients, can be found completely and persistently in the PSD group rats. With good operability and repeatability, the rats PSD model is an ideal model for the PSD research. Fluoxetine can improve the behavior abnormality of the PSD rats.

Key words: Stroke; Depressive disorder; Models; Animal; Fluoxertin

摘要： 目的 建立卒中后抑郁（poststroke depression，PSD）有效动物模型。方法 大脑中动脉闭塞（MCAO）制备大鼠局灶脑缺血模型，加以慢性不可预见的温和刺激结合孤养建立PSD模型并予氟西汀干预。分为对照组、卒中组、抑郁组、PSD组和PSD+氟西汀组。分别采用糖水消耗试验、旷野试验（open-field test，OFT)、强迫游泳评估大鼠快感缺失、活动减少、行为绝望等行为。结果 应激14 d时，与对照组及卒中组相比，PSD组体重增长幅度显著降低（P<0.05），经氟西汀干预后体重增长幅度明显增加（P<0.01）。PSD组水平得分在应激第7天时与对照组相比显著降低（P<0.05）；到应激14 d时，PSD组与对照组及卒中组相比水平得分进一步下降（P<0.01），并持续到应激18 d（P<0.01）。PSD组垂直得分在应激14 d时与对照组、卒中组相比均显著下降（P<0.05或P<0.01），强迫游泳的不动时间明显延长（P<0.05）；而氟西汀干预后水平得分与垂直得分均显著增加（P<0.05或P<0.01），不动时间明显缩短（P<0.01）。结论 PSD模型大鼠较充分而持续表现快感缺乏、活动减少等“抑郁”核心症状，可操作性和重复性较好，是研究PSD较为理想的大鼠模型。氟西汀能改善PSD模型大鼠行为学异常。

关键词:   卒中; 抑郁; 模型; 动物; 氟西汀