Abstract: Objective To investigate the potential cell types for neuroprotection of DL-3-n-butylphthalideagainst ischemic brain injury.Methods The Oxygen-Glucose Deprivation- Reperfusion models were created in rat adrenalpheochromocytoma (PC12) cells and rat artery endothelial cells (SUVRAEC) and the effect of DL-3-n-butylphthalide on stroke in different cell types was assessed using 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) assay. The direct free-radical scavenging ability ofDL-3-n-butylphthalide was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay.Results Different concentrations of DL-3-n-butylphthalide (1/10/100μmol/L) did not have anynegative effect on cell viability. DPPH assay did not reveal any direct free-radical scavengingability of DL-3-n-butylphthalide. DL-3-n-butylphthalide concentration-dependently attenuatedOGD-induced decrease in the cell viability in SUVRAEC but not neuron-like PC12 cells. DL-3-nbutylphthalideat an effective concentration could rescue 15% more cells than groups without it andapproximate 90% SUVRAEC in total were alive.Conclusion DL-3-n-butylphthalide selectively protects SUVRAEC against ischemic injury anddoes not have a direct free radical scavenging property.

Key words: DL-3-n-butylphthalide; Stroke; Neuron; Endothelial cells

摘要： 目的 研究丁苯酞对缺血性脑损伤产生作用的准确细胞类型。方法 分别使用大鼠肾上腺髓质嗜铬细胞瘤细胞（Rat adrenal pheochromocytoma，PC12）和大鼠动脉内皮细胞（rat artery endothelial cells，SUVRAEC）构建氧气葡萄糖剥夺-再灌注损伤模型，通过噻唑蓝［3-（4，5-dimethylthiazole-2-yl）-2，5-biphenyl tetrazolium bromide，MTT］进行细胞活性检测，观察单纯损伤细胞与各剂量丁苯酞处理后的细胞活性。同时，采用1，1-二苯基-2-苦基偕腙肼（1，1-diphenyl-2-picrylhydrazyl，DPPH）法检测自由基清除活性。结果 丁苯酞各剂量组对PC12和SUVRAEC的细胞活性均无明显影响，均不能抑制自由基离子。丁苯酞处理使经受氧气葡萄糖剥夺-再灌注损伤的SUVRAEC细胞活性增加约15%，约90%的细胞活性得到有效保护，并具有剂量依赖性；而对PC12的细胞活性则无明显作用。结论 丁苯酞对PC12和SUVRAEC均无毒性作用，不具有直接清除自由基的能力；丁苯酞主要作用于血管内皮细胞，而对神经元的作用相对较弱。

关键词: 丁苯酞; 卒中; 神经元; 内皮细胞