Objective To explore the expression of glutamate interacting protein (GRIP) and its relationshipwith glutamate receptor 2 (GluR2) after ischemia/reperfusion in rats brain.Methods Thirty-six male Sprague Dawley rats were randomly divided into ischemia-reperfusiongroup and sham group. Middle cerebral artery occlusion (MCAO) was achieved with the use of anintraluminal filament to occlude the left MCA. Then the reperfusion was applied for different lengthof time. Sham-operations were also done (n=6). At 1, 6, 12, 24 and 72 hour after reperfusion, allrats were decapitated. Western blot was used to determine the expression of GRIP, GluR2 on themembrane and in the cytoplasm.Results Western blot showed that at 1h, 6h, 12h, 24h and 72h after reperfusion, the ratio of GRIPin operation group to that in sham group were from 1.11±0.21, 1.42±0.03, 1.49±0.03, 1.31±0.03 and1.20±0.03. It showed significant increase (P <0.01) compared with sham except 1h group. The ratioof GluR2 on the membrane to that in the cytoplasm were 1.38±0.12, 1.78±0.11, 3.53±0.15, 4.62±0.24and 0.87±0.08 respectively. It also exhibited significant than sham group (P <0.01) except 1h group.Conclusion Both GRIP and GluR2 on the membrane increased obviously after ischemiareperfusion,which may take an important role in the AMPA receptor mediated excitotoxic injury.【
