关键词: 丹酚酸B; 脑缺血; 再灌注损伤; 病理形态; 细胞凋亡

Abstract: Objective To investigate the histopathological change of brain tissue after focal cerebral ischemia reperfusion injury in rats and the neuroprotective effect of Sallvianolic acid B.Methods Thirty-two healthy male Wistar rats were randomly divided into 4 groups with 8 rats in each group: sham-operation group, model group, middle-dosage group and high-dosage group of salvianolic acid B. Distilled water was given to the fore two groups by intragastric administration route. Different Salvianolic acid B concentration, 96 mg/kg and 192 mg/kg ,was given to the latter groups by same way respectively. All groups were given once daily for 7 days. One hour after last infusion, middle cerebral artery occlusion(MCAO)was induced using intraluminal suture technique in the rats except the sham-operation group. After 2 h of ischemia and followed 24 h of reperfusion, the pathomorphological changes of brain tissue in all groups of rats were observed using hematoxylin and eosin(HE) staining method, and the ischemic area of brain tissue was detected using pathological image analytical technique and apoptotic index was detected using terminal uridine deoxynucleotidyl transferase nick end labeling(TUNEL) method.Results In model group,compared with sham-operation group, we could find that the focal ischemic regions, disordered tissue construction,interstitial substance edema,neuron degeneration and necrosis were severe in brain tissue of the injury side and apoptotic indexes were increased. In both Salvianolic acid B groups,compared with model group,disordered construction of ischemic regions, interstitial substance edema and the degree of neuron degeneration were relieved and necrosis cells were obviously decreased, the cerebral ischemic area was significantly decreased by 12% and 14% respectively(P<0.01), and apoptotic indexes were also significantly decreased by 16.0% and 17.3% respectively(P<0.01), but there was no dose-dependent relation.Conclusion .Salvianolic acid B has protective effect on histomorphology of brain tissue in rats with focal cerebral ischemia reperfusion injury.

Key words: Salvianolic acid B; Cerebral ischemia; reperfusion injury; Histopathology; Apoptosis