关键词: 脑梗死; 肿瘤坏死因子-α; 脑水肿; 大鼠

Abstract: Objective To investigate the effects of Bio-wave regulation factor (BRF) on tumor necrosis factor- α(TNF-α) expression in rats after middle cerebral artery occlusion (MCAO). Methods A total of 90 adult male Sprague-Dawley rats were randomly divided into sham operation group, normal saline group and BRF treatment group. Each group consisted of five subgroups (6h, 24h, 48h, 72h and 7d), according to different time points post-ischemia. Rats in BRF treatment group and normal saline group were subjected to MCAO and administrated intraperitoneally with 1.25% BRF solution (1ml/100g) and normal saline (1ml/100g) respectively sixty minutes after operation, then once a day till they were sacrificed. Four different methods were used for assessing neurological deficit score (NDS) , the wet-dry method for measuring brain water content, hematoxylin and eosin(HE) staining technique for observing histopathological changes, immunohistochemistry method for detecting TNF-α expression. Results 1. Behavioral evaluation: the behavioral outcomes were most serious at 48h, 72h and back to normal at 7d after MCAO. The NDS was lower in BRF treatment group than that of normal saline group at 48h. 2. Brain water content: Brain water contents both in normal saline group and BRF treatment group increased obviously at any points. Compared with normal saline group, brain edema in BRF treatment group was attenuated apparently, especially at 48h (P <0.05). 3. HE staining: histopathologic damages were alleviated with BRF administration, the inflammatory cell infiltration increased at 6h, peaked at 48h, and continued to 7d. 4. The expression of TNF-α: compared with normal saline group, the expression of TNF-α in BRF treatment group attenuated obviously at 48h and 72h (P <0.05). Conclusion The results suggest that BRF may produce neuroprotective effects against infarction damage by reducing brain edema and inhibiting the expression of TNF-α.

Key words: Cerebral infarction; Tumor necrosis factor –α(TNF-α); Brain edema; Rat