厄贝沙坦对溶血磷脂酰胆碱所致人静脉内皮细胞损害的影响

›› 2008, Vol. 3 ›› Issue (10): 721-725.

厄贝沙坦对溶血磷脂酰胆碱所致人静脉内皮细胞损害的影响

戴文卓，石静萍，林兴建

南京医科大学附属南京脑科医院神经内科（戴文卓现在江苏省无锡市第二医院）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-10-20 发布日期:2008-10-20
通讯作者: 石静萍

The Study of Protection of Irb on Human Umbilical Vein Endothelial Cells Impaired by Lysophosphatidylcholine

DAI Wen-Zhuo, SHI Jing-Ping, LIN Xing-Jian.

Department of Neurology of Nanjing Brain Hospital affiliated to Nanjing Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2008-10-20 Published:2008-10-20
Contact: SHI Jing-Ping

摘要/Abstract

摘要： 目的观察厄贝沙坦（Irbesartan，Irb）对由溶血磷脂酰胆碱（Lysophosphatidyl choline，LPC）所致人脐静脉内皮细胞（Human umbilical vein endothelial cells，HUVECs）损害的影响。方法体外培养HUVECs，分为（1）正常对照组；（2）低浓度LPC组（10μg/L）；（3）中浓度LPC组（20μg/L）；（4）高浓度LPC组（40μg/L）；（5）Irb对照组（含LPC20μg/L）；（6）低浓度Irb组(10-7μmol/L+LPC20μg/L)；（7）中浓度Irb组(10-6μmol/L+LPC20μg/L)；（8）高浓度Irb组(10-5μmol/L+LPC20μg/L)。采用放免及RT-PCR法分别观测LPC对HUVECs血管紧张素II(Angiotensin II，ATII)蛋白及AT1型受体（Angontensin II 1 receptor，AT1R）、AT2型受体（Angontensin II2 receptor，AT2R）mRNA（messenger Ribonucleic Acid）的表达的影响；采用化学比色法测定一氧化氮（NO）含量，一氧化氮合酶（NOS）及超氧化物歧化酶（SOD）活性；并观测使用Irb干预后的效果。结果与正常对照组相比，LPC使HUVECs ATII蛋白及AT1RmRNA的表达显著增加，使NO含量、NOS及SOD活性显著下降；经Ibr干预后显著增加了HUVECs NO含量、NOS及SOD的活性。结论 Irb可以对LPC所致的内皮细胞损害发挥部分保护作用。

关键词: 厄贝沙坦; 溶血磷脂酰胆碱; 脐静脉; 内皮细胞

Abstract: Objective To investigate the protection effect of irbesartan on cultured human umbilical vein endothelial cells(HUVECs) impaired by lysophosphatidylcho-line(LPC).Methods HUVECs were cultured in vitro. The study was designated to 8 groups: (1)normal control, (2)low-concentration LPC group (10μg/L), (3)middle-concentration LPC group(20μg/L), (4) high-concentration LPC group (40μg/L), (5)Irb control group containing LPC(20μg/L),(6)low-concentration Irb group(10-7μmol/L),(7) middle-concentration Irb group(10-6μmol/L) ,(8) high-concentration Irb group(10-5μmol/L). Expression of angiotensin II (ATII) and ATII type1 receptor(AT1R), AT type2 receptor(AT2R) mRNA were observed by radio-immunity and RT-PCR respectively. Production of nitrogen monoxidum (NO), activity of nitricoxide synthase (NOS) and superoxide dismutase (SOD) were determined by chromatometry. Also the intervention effect of Irb was observed.Results Compared with normal control group, LPC could significantly up-regulate the expression of ATII and AT1R mRNA and decrease the expression of NO and the activity of NOS,SOD in a dose-dependent manner; Irb could significantly increase the expression of NO and the activity of NOS and SOD.Conclusion Irb could partly protect HUVECs function against the LPC-induced damage.

Key words: Irbesartan; Lysophosphatidylcholine; Umbilical veins; Endothelial cells

戴文卓;石静萍;林兴建. 厄贝沙坦对溶血磷脂酰胆碱所致人静脉内皮细胞损害的影响[J]. , 2008, 3(10): 721-725.

DAI Wen-Zhuo;SHI Jing-Ping;LIN Xing-Jian.. The Study of Protection of Irb on Human Umbilical Vein Endothelial Cells Impaired by Lysophosphatidylcholine[J]. , 2008, 3(10): 721-725.

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