关键词: 人脐血单核细胞; 脑出血; 大鼠; 神经功能

Abstract:

【Abstract】Objective To observe the changes in behavior in rats with cerebral hemorrhage which were transplanted human umbilical cord blood stem cells by different ways in two weeks and to discuss the best pathway of stem cell transplanted.Methods An animal model of intracerebral hemorrhage(ICH) was established by autologous blood injection/withdraws two times. The success of cerebral hemorrhage model confirmed by computed tomography(CT) examination. And then fresh human umbilical cord blood mononuclear cells(HUCBMC) which were isolated from human umbilical cord blood were transplanted into experimental animal in vivo respectively by Wister rats, caudal vein, left ventricular and theposition of cerebral hemorrhage. The control group was fed well without treatment after modeling successfully and observed the process of natural recovery. The behavior of each model was assessed by Longa 5 grading method on 1, 3, 7 and 14 days.

Results Statistical data suggested that it was a significant difference in the changes of rats,neurological function at different time points which were transplanted HUCBMC through the tail vein, left ventricular and cerebral hemorrhage local pathways(F =131.87, P ＜0.001). The factors which time and group interaction effect showed that different treatment tend to the same trends of time(F =35.54, P ＞0.05). Influence of different transplantation methods on changes of neural function in rats was different(F =6.434, P =0.001). The rats, neurological function scores(2.35±0.67) which were transplanted HUCBMC by cerebral hemorrhage local pathways were obviously higher than the other groups on the 1st day. And the scores(0.40±0.60, 0.25±0.37, 0.03±0.22) were lower than the other groups on the 3th, 7th and 14th days. There was no significant differences between the neurological function scores of the four groups when HUCBMC were transplanted for 1 day(F =2.14, P =0.10). There were significant differences between the group transplanted by the local cerebral group and the other groups on the 3th, 7th and 14th days(F =5.59, 22.94 and 11.07, All of P ＜0.01).There were no significant differences between the group transplanted by caudal vein and left ventricular(P =0.85, 0.08, 0.70, 0.68).

Conclusion Human umbilical cord blood stem cell transplantation can improve the neural function in rats with cerebral hemorrhage through the tail vein, left ventricular and cerebral hemorrhage local pathway. The best way of transplantation was local cerebral transplantation.

Key words: Human umbilical cord blood stem cells; Cerebral hemorrhage; Rat; Neuralfunction