磁共振在大鼠脑缺血再灌注模型中的应用研究

摘要/Abstract

摘要：

【摘要】目的利用磁共振成像（magnetic resonance imaging，MRI）筛选合格的大鼠大脑中动脉闭塞（middle cerebral artery occlusion，MCAO）模型，分析大鼠脑缺血再灌损伤后表观弥散系数（apparent diffusion coefficient，ADC）及部分各向异性（fractional anisotropy，FA）指标的变化特征。方法制作SD大鼠MCAO模型，根据是否再灌注分为永久闭塞组和缺血再灌组，每组各9只，另设假手术组2只作为对照。对大鼠行磁共振T2、弥散加权成像（diffusion weighted imaging，DWI）、弥散张量成像（diffusion tensor imaging，DTI）和磁共振血管成像（magnetic resonance angiography，MRA）扫描，重建脑血管及计算相对ADC值和FA值，比较两组的相对ADC值和FA值。结果 MRA可以显示大鼠脑血管的堵塞与再通，T2加权像可以观测到蛛网膜下腔出血。永久闭塞组比缺血再灌组的相对ADC值高，差异有显著性（0.657±0.140 vs 0.588±0.132，t＝2.27，P=0.048）；相对FA值高，差异有显著性（1.010±0.143 vs 0.915±0.111，t＝2.89，P=0.02）。结论利用MRA和T2加权像扫描可以筛选出合格的MCAO模型，MCAO永久闭塞组相对ADC值和FA值均高于缺血再灌注组。

文章导读： 国内首次应用3.0T磁共振成像筛选大鼠大脑中动脉永久闭塞和缺血再灌注模型，并比较了两者的磁共振和病理学特点。

关键词: 磁共振血管成像; 表观弥散系数; 部分各向异性; 脑缺血再灌注损伤

Abstract:

【Abstract】 Objective To screen the stable middle cerebral artery occlusion (MCAO) model by magnetic resonance angiography (MRA) and T2 scan, to analyze the characteristics of apparent diffusion coefficient (ADC), fractional anisotropy (FA) change after ischemia-reperfusion injury. Methods The MCAO model of SD rat was made. Those rats were divided into sham-operation group, permanent middle cerebral artery occlusion (pMCAO) group, and transient middle cerebral artery occlusion (tMCAO) group according to cerebral artery reperfusion and occlusion. Each experiment group has nine rats, and the control group has 2 rats. MRA T2, diffusion weighted imaging (DWI), diffusion tensor imaging (DTI) by 3.0-T magnetic resonance imaging (MRI) clinical scanner were applied when the unilateral middle cerebral artery was occluded and reopened. After scanning, reconstruct the cerebral vessel and calculate the relative ADC value and FA value, and compare relative ADC value and FA value of two groups. Results The occlusion and reperfusion of rat's cerebral vessel was clearly demonstrated by MRA and subarachnoid hemorrhage was observed on T2. Intensity signal in maps of ADC and FA of pMCAO was higher than that of tMCAO(0.657±0.140 vs 0.588±0.132, trADC=2.27, P=0.048; 1.010±0.143 vs 0.915+0.111, trFA=2.89, P=0.02). Conclusion The stable MCAO model can be screened out by MRA and T2, and rADC value and rFA values of pMCAO group are higher than those of tMCAO group.

Key words: Magnetic resonance angiography; Apparent diffusion coefficient; Fractional anisotropy; Cerebral ischemia-reperfusion injury

韩伟1，秦文1，薛蓉2，于春水1，张云亭1. 磁共振在大鼠脑缺血再灌注模型中的应用研究[J]. 中国卒中杂志, 2013, 8(06): 454-458.

HAN Wei*, QING Wen, XUE Rong, YU Chun-Shui, ZHANG Yun-Ting.. Application of Magnetic Resonance Imaging on Ischemia-reperfusion Rat Models[J]. Chinese Journal of Stroke, 2013, 8(06): 454-458.

参考文献

Kidwell CS, Jahan R, Gornbein J, et al. A trial of imaging selection and endovascular treatment for ischemic stroke[J]. N Engl J Med, 2013, 368:914-923.

Gerriets T, Stolz E, Walberer M, et al. Complications and pitfalls in rat stroke models for middle cerebral artery occlusion:a comparison between the suture and the macrosphere model using magnetic resonance angiography[J]. Stroke, 2004, 35:2372-2377.

赵凯, 张国华, 贾宇臣, 等. 线栓法制备大鼠局灶性脑缺血/再灌注模型的改良及探讨[J]. 中国卒中杂志, 2012, 7:916-921.

Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20:84-91.

Gerriets T, Stolz E, Walberer M, et al. Noninvasive quantification of brain edema and the space-occupying effect in rat stroke models using magnetic resonance imaging[J]. Stroke, 2004, 35:566-571.

Zhang W. Role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury[EB/QL]. PLoS One(2013-4-9)[2013-4-20]. http://www.10.1371/journal.pone.0061244.Besselmann M, Liu M, Diedenhofen M, et al. MR angiographic investigation of transient focal cerebral ischemia in rat[J]. NMR Biomed, 2001, 14:289-296.

汪有锦, 姚群立, 滕皋军, 等. 7.0 T磁共振灌注和弥散加权成像的缺血半暗带实验研究[J]. 中华医学杂志, 2010, 90:1773-1777.

Yang YM, Feng X, Yao ZW, et al. Magnetic resonance angiography of carotid and cerebral arterial occlusion in rats using a clinical scanner[J]. J Neurosci Methods, 2008, 167:176-183.

Wegener S, Weber R, Ramos-Cabrer P, et al. Temporal profile of T2-weighted MRI distinguishes between pannecrosis and selective neuronal death after transient focal cerebral ischemia in the rat[J]. J Cereb Blood Flow Metab, 2006, 26:38-147.

杨艳梅, 冯晓源, 崔梅, 等. MRI对线栓法制作大鼠脑缺血再灌注模型分组的价值[J]. 临床放射学杂志, 2007, 26:917-920.

Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury[J]. J Pathol, 2000, 190:255-266.

Liu X, Tian W, Li L, et al. Hyperintensity on diffusion weighted image along ipsilateral cortical spinal tract after cerebral ischemic stroke:a diffusion tensor analysis[J]. Eur J Radiol, 2012, 81:292-297.

Duncan JR. Adolescent toluene inhalation in rats affects white matter maturation with the potential for recovery following abstinence[EB/QL]. PLoS One(2012-9-18)[2013-4-20]. http://www.10.1371/journal.pone.0044790.

Adluru N, Zhang H, Fox AS, et al. A diffusion tensor brain template for Rhesus macaque[J]. Neuroimage, 2012, 59:306-318.

许强, 张云亭. 超急性与急性期脑缺血半暗带演变的DWI研究[J]. 中国医学影像技术, 2007, 23:52-55.

[1]	王恺闻, 卢宝全, 吴秀玲, 马佳丽. 基于MRA评估基底动脉闭塞预后评分预测非重症基底动脉闭塞患者预后效能的研究 [J]. 中国卒中杂志, 2022, 17(12): 1335-1342.
[2]	毕京凤, 李琳琳, 刘欣瑶, 张英魁. 颈部动脉夹层磁共振成像多序列评估与信号解读[J]. 中国卒中杂志, 2022, 17(04): 402-406.
[3]	李昕, 张英魁. 扩散加权成像中几个相关概念与图像解读[J]. 中国卒中杂志, 2022, 17(02): 195-199.
[4]	王一轻, 刘平国, 沈佳慧, 蔡增林. 脑穿支动脉病变梗死类型和神经影像学研究进展[J]. 中国卒中杂志, 2021, 16(09): 877-882.
[5]	隋滨滨;高培毅;林燕;薛静;沈宓;王辉;王琼;. 三维时间飞跃法磁共振血管成像原始图像评估脑微出血病灶的应用价值[J]. , 2012, 7(06): 466-471.
[6]	王艳;王伊龙;吴敌;王拥军. 颅内动脉中重度狭窄的危险因素研究[J]. , 2007, 2(06): 32-36.