关键词: 星形胶质细胞; 脑缺血; 分布; 大鼠

Abstract: Objective To observe the temporal and spatial distributions of astrocytes after permanent localcerebral ischemia.Methods Total 54 male SD rats were selected and randomly divided into 2 groups, sham-operationgroup and operation group. Depending on the time point from 3 hours to 48 hours, the operationrats were divided into 5 subgroups. Animal model of focal cerebral ischemia was established bynylon suture embolization and cerclage. The brain samples of each group were obtained at 3 h, 6 h,12 h, 24 h and 48 h after embolization, respectively. Morphologic changes after hematoxylin andeosin(HE) dying, TdT-mediated dUTP-biotin nick end labeling (TUNEL) and glial fibrillary acidicprotein(GFAP) immunohistochemistry analysis were observed. Imaging analysis technique wasused to detect the temporal and spatial distributions of the astrocytes in each animal.Results As ischemic condition continued, both TUNEL and GFAP positive cells decreased in the corearea of cerebral ischemia. And in the marginal area, the TUNEL positive cells increased with timepassing by, while at 12-hour time point, the GFAP positive cells decreased mostly and then increasedslowly.Conclusion At the time point of 12 hour of cerebral ischemia in the marginal area, the temporal andspatial distributions of astrocytes are not coincided with those of astrocytes in core area, which maymean that during different period of cerebral ischemia, astrocytes may play different roles.

Key words: Astrocyte; Cerebral ischemia; Distribution; Rat