关键词: 表皮生长因子; 神经元可塑性; 脑梗死

Abstract: Objective To investigate the effect of epidermal growth factor (EGF) on neuronal recovery in adultrats with cortical ischemic infarctions.Methods One day after the right middle cerebral artery occlusion (MCAO) in renovascularhypertensive rats, 10 μl EGF (100μg /L ) was infused consecutively into lateral ventricle in 32experimental rats ( EGF group) and the same amount of vehicle in 32 MCAO rats as controls for2 days. At the end of 1, 2, 3, and 4 weeks after the MCAO, the forebrains of eight rats from eachgroup respectively were taken and homogenated for the detection of growth-associated protein43(GAP43), synaptophysin(SYN), and glial fibrillary acidic protein(GFAP) by western blotfollowing Bederson neurological evaluation. Sham controls were matched at the baseline at eachtime.Results Earlier and higher peak expressions of GAP43, SYN, and GFAP in the hemisphereipsilateral to infarct cavity, with better neurological function at the end of 1, 2 weeks after theMCAO, were found in EGF group than in vehicle group (P < 0.05).Conclusion The increased expression of GAP43, SYN, and GFAP is related to early neuralrecovery following EGF infusion after stroke.

Key words: Epidermal growth factor; Neural plasticity; Cerebral infarction