关键词: 趋化因子; 基因多态性; 脑梗死; TOAST分型

Abstract: Objective To investigate the relationship between chemokine CXC ligand 16(CXCL16) gene A181V polymorphism, serum CXCL16 levels and cerebral infarction subtypes according to the trial of ORG 10172 in acute stroke treatment (TOAST) classification.Methods The polymorphisms of CXCL16 gene A181V were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The serum CXCL16 levels in 74 healthy controls and 177 patients with acute cerebral infarction were measured by Enzyme linked immunosorbent assay(ELISA).Results The serum CXCL16 levels of patients were significant higher than those of healthy controls(P<0.001). According to TOAST classification, the patient controls were divided into three groups. Then we found that serum CXCL16 levels were significant different among large artery atherosclerotic stroke(LAA) group, small artery occlusion stroke(SAO) group and healthy controls(P<0.001). Logistic regression revealed that serum CXCL16 levels were an independent risk factor for cerebral infarction(P<0.001). The CXCL16 gene A181V genotype distribution and allele frequencies showed no differences between patient controls and healthy controls(P>0.05). However, among LAA group, SAO group and healthy controls the AAhomozygotes had significantly higher cerebral infarction risk compared with the G allele carriers (GA+GG)(P<0.001). CXCL16 gene A181V polymorphism was associated to serum CXCL16 levels(P=0.009).Conclusions Serum CXCL16 level correspond well with CXCL16 gene A181V polymorphism. An allele may be useful to identify patients at increased risk of Large artery atherosclerotic stroke.

Key words: Chemokine; Gene polymorphism; Cerebral infarction; TOAST classification