关键词: 线栓法; 脑缺血; 再灌注; 动物模型

Abstract:

【Abstract】 Objective To establish a simple, reliable and stabilized rat model of local cerebral ischemia/reperfusion model in rats. Methods The local cerebral ischemia/reperfusion model in rats was set up by referring to Zea Longa method. Sixty Wistar rats were randomly divided into 2 groups(30 cases in modified group, 30 cases in traditional method group). Modified group mainly focused on three aspects to improve the success rate, involved the treatment of the line bolt head, method of how to insert the intraluminal suture and selection of insertion position etc. Stability and the success rate of model were detected by behavioral test of neurological deficit scoring, magnetic resonance imaging(MRI) inspection and pathological examination. Results Neurological function score of the modified group(2.35±1.23) points had significant difference compared with the traditional group(1.24±1.50) points(t=2.852, P=0.007), the success rate of survival animal model and experimental animal model were different significantly between the modified group(73.08%, 63.33%) and traditional group(33.33%, 20.00%)(χ2=11.589 & 6.848, P=0.001 & 0.02). Conclusions Neurological scores and success rate of modified group model was significantly higher than the traditional group. It is an ideal making model approach of local cerebral ischemia/reperfusion.

Key words: Suture-occluded method; Ischemia; Reperfusion; Animal model