关键词: 门冬氨酸钾; 脑缺血; 凋亡

Abstract:

Objective To investigate the protection of potassium aspartate (PA) against focal cerebral ischemia/ reperfusion in rats. Methods Male Sprague-Dawley rats were subjected to 2 h of right middle cerebral artery occlusion (MCAO) and 22 h of reperfusion. Rats were randomly divided into 5 groups, 10 rats in each group. Saline (1 ml/kg) or different doses of PA (10 mg/kg, 25 mg/kg, 62.5 mg/kg and 125 mg/kg) was administrated intraperitoneally after 1 h MCAo, to observe the effects of PA at different dosage on neurological deficits and infarct volume after cerebral ischemia/reperfusion in rats. Another 32 rats were assigned to vehicle group and PA group randomly, at 1 h after ischemia by intraperitoneal injection of saline (1 ml/kg) or PA (62.5 mg/kg). Sixteen rats were used as the sham operation group. Brain adenosine triphosphate (ATP) and lactic acid levels (n =10), and the apoptotic cell death (n =6) were evaluated. Results PA treatment at the dose of 62.5 mg/kg significantly improved neurological deficits (P <0.001) and decreased the infarct volume compared with vehicle treatment (P =0.011); compared with vehicle treatment, PA treatment at the dose of 25 mg/kg dose can reduce the infarct volume markedly (P =0.040), but no differences in neurological deficits; both neurological deficits and infarct volume were improved following 10 mg/kg and 125 mg/kg PA in comparison to vehicle, although it was not significant. PA treatment (62.5 mg/kg) significantly reduced the loss of ATP (P =0.036), and the apoptotic cell death (P <0.001) compared with vehicle treatment. Conclusion PA has neuroprotective effects against apoptosis after cerebral ischemia/reperfusion in rats.

Key words: Potassium aspartate; Cerebral ischemia; Apoptosis