消栓肠溶胶囊对大脑中动脉栓塞大鼠远
隔脑区星形胶质细胞活化及凋亡蛋白表
达的影响

摘要/Abstract

摘要：

目的观察消栓肠溶胶囊对大脑中动脉栓塞大鼠远隔脑区星形胶质细胞活化及凋亡蛋白半胱氨酸天冬氨酸蛋白酶（cystein-aspartate protease 3，Caspase-3）及其底物多聚腺苷酸二磷酸核糖聚合酶（poly ADP-ribose polymerase，PARP）表达的影响。方法线栓法建立大脑中动脉栓塞模型，雄性SD大鼠90只随机分成假手术组、模型组、消栓肠溶胶囊（420 mg/kg、140 mg/kg、47 mg/kg）组、阳性对照药脑心通胶囊（600 mg/kg）组，每组15只，连续灌胃给药15 d，每天给药1次。分别在术后3 d、7 d、12 d、15 d检测前肢抓握力量；术后15 d采用免疫荧光染色结合图像分析技术检测海马胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）和 Caspase-3、PARP的表达。结果大鼠大脑中动脉栓塞第3天、第7天、第12天、第15天前肢抓握力量均较同时间段假手术组大鼠减小，差异具有显著性（P＜0.01）；大脑中动脉栓塞第15天，大鼠远隔脑区海马GFAP、PARP表达增强，Caspase-3阳性细胞增多，差异均较假手术组明显（P＜0.05）。消栓肠溶胶囊420 mg/kg治疗后，大脑中动脉栓塞第7天大鼠前肢抓握力量增加；消栓肠溶胶囊140 mg/kg组大鼠大脑中动脉栓塞第12 天前肢抓握力量增强；大脑中动脉栓塞第15天，消栓肠溶胶囊420 mg/kg、140 mg/kg、47 mg/kg组和脑心通胶囊组大鼠前肢抓握力量均提高，差异均较模型组显著（P＜0.05）。和模型组相比，消栓肠溶胶囊（420 mg/kg、140 mg/kg、47mg/kg）组大鼠海马GFAP、PARP阳性表达强度降低（P＜0.01）， Caspase-3阳性细胞数减少，差异均较模型组明显（P＜0.05或P＜0.01）。脑心通胶囊也可明显下调大鼠海马GFAP、PARP表达，和模型组比较差异具有显著性（P＜0.01）。结论消栓肠溶胶囊可通过抑制星形胶质细胞异常活化，抑制Caspase-3、PARP凋亡信号分子激活，保护缺血远隔脑区。

文章导读： 本研究发现消栓肠溶胶囊可抑制星形胶质细胞异常活化及Caspase-3、PARP表达，保护缺血远隔
脑区，改善缺血动物模型的行为学功能

关键词: 消栓肠溶胶囊; 脑缺血; 多聚腺苷酸二磷酸核糖聚合酶; 半胱氨酸天冬氨酸蛋白酶; 胶质纤维酸性蛋白

Abstract:

Objective To investigate the effects of Xiaoshuan enteric-coated capsule on astrocyte activation and expression of cystein-aspartate protease 3 (Caspase-3), poly ADP-ribose polymerase (PARP) in middle cerebral artery occlusion (MCAO) rats of remote encephalic region. Methods The study adopted the animal model of MCAO. Xiaoshuan enteric-coated capsule (420, 140, 47 mg/kg) was given orally to rats once each day for 15 d after ischemia. Bar-grasping was measured at 3 d, 7 d, 12 d and 15 d after ischemia. The expression of glial fibrillary acidic protein (GFAP), Caspase-3 and PARP were detected by using immunofluorescence at 15 d after ischemia. Results Xiaoshuan enteric-coated capsule strengthened the ability of bar-grasping of MCAO rats at 7 d, 12 d and 15 d after ischemia (P <0.05). In addition, Xiaoshuan enteric-coated capsule significantly controlled the positive expression of GFAP and PARP (P <0.01), decreased the numbers of Caspase-3 positive cells (P <0.05) in hippocampus of MCAO rats. Conclusion Xiaoshuan enteric-coated capsule reduces the activation of GFAP, Caspase-3 and PARP. As a result, Xiaoshuan enteric-coated capsule can protect the remote encephalic region of ischemia.

Key words: Xiaoshuan enteric-coated capsule; Cerebral ischemia; Poly (ADP-ribose)
polymerase; Cystein-aspartate protease3 (caspase3); Glial fibrillary acidic protein

王雅丽1，张宁2，刘菁2，吴曦2，王蕾1，赵晖1. 消栓肠溶胶囊对大脑中动脉栓塞大鼠远
隔脑区星形胶质细胞活化及凋亡蛋白表
达的影响[J]. 中国卒中杂志, 2014, 9(10): 824-830.

WANG Ya-Li*, ZHANG Ning, LIU Jing, WU Xi, WANG Lei, ZHAO Hui.. Effects of Xiaoshuan Enteric-coated Capsule on Astrocyte Activation and Expression of
Caspase-3, PARP in Remote Encephalic Region of MCAO Rats[J]. Chinese Journal of Stroke, 2014, 9(10): 824-830.

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