关键词: 脑源性神经营养因子; 脑缺血; 高血压

Abstract: Objective To study whether hypertension may affect the expression of brain-derived neurotrophicfactor in the hippocampus and cerebral cortex after chronic cerebral ischemia.Methods Normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR)received permanent occlusion of bilateral common carotid arteries (chronic ischemic animalmodel). In situ hybridization and immunohistochemistry were used to detect the changes ofbrain-derived neurotrophic factor at 1 week and 4 weeks after chronic ischemia. H&E stain wasused to examine the cerebral infarct volume at 4 weeks after ischemia.Results There was a significant reduction of brain-derived neurotrophic factor mRNA andimmunoreactivity in the hippocampal CA1 and cerebral cortex of SHR from 1 week to 4 weeksafter chronic ischemia (P <0.05). Western blot study gave the same result. However, WKY ratshad only transient decrease at 1 week after ischemia (P <0.05). H&E stain showed there was asignificantly larger area of brain infarct in the SHR than WKY rats (12.40±4.26% vs 0.41±0.17%,P =0.026).Conclusion Our study indicates that under chronic ischemia, hypertension may aggravatethe ischemic brain damage and influence the expression of brain-derived neurotrophic factorespecially in ischemia-vulnerable hippocampal CA1 and cerebral cortex.

Key words: Brain-derived neurotrophic factor; Brain infarction; Hypertension